The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned right into a pET21a+ vector working with NdeI and XhoI restriction enzymes. A degree mutation was introduced by the https://englandr998epa1.bimmwiki.com/user